SHST - Horticulture and Forestry Society from Transylvania, 40 Years of Horticulture Education in Cluj-Napoca

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Effect of Selected Cultivation Factors on the Growth of Mycelium of Pleurotus cystidiosus Miller

Last modified: 2017-04-18


Pleurotus cystidiosus occurs in natural areas in North America. Its carpophores are characterized by slightly sweet and mild taste. Fruiting bodies of P. cystidiosus are valuable sources of nutrients and biologically active substances. Selection of the best agar medium, optimal incubation temperature and the best substrate for properly mycelium growth remain important aims. Two cultivars of P. cystidiosus – ‘B1’ and ‘B122’ and the growth of mycelium on five agar media (wheat, potato, maltose, sawdust and Hansen’s) were studied. Incubation of mycelium was performed at 15 to 30 °C. The influence of substrate was analysed using wheat straw, hemp shives and energetic grass – Panicum virgatum. In the experiment there was compared growth of mycelium on substrate with wheat straw (control sample) and with wheat straw and addition of hemp shives and energetic grass, in growing amount: 10, 20 and 30%. The results shown that the growth of mycelium of tested strains on different agar media and different substrates was various. Due to a faster growth of mycelium of P. cystidiosus, wheat and sawdust agar media show a greater ability to be used on a large scale of cultivation. The best growth of mycelium occurred on substrate with wheat straw and addition of hemp shives in an amount of 20% and on substrate with wheat straw and addition of Panicum virgatum in an amount of 30%. Temperature of incubation had also a significant impact on the growth of mycelium of P. cystidiosus, the optimal temperature being 25 °C. Investigation of mutual dependence between morphological and qualitative characteristics of type of agar medium allows optimization of production of P. cystidiosus. Development of low-cost and simple method for production of P. cystidiosus can contribute to introduction of this species into intensive cultivation. Use of waste materials in mushroom production will significantly increase profitability of crops and reduce its costs.



This abstract was accepted to be presented at the conference entitled “40 Years of Horticulture Education in Cluj-Napoca” Cluj-Napoca, September 27, 2017, being included in ‘Book of Abstracts’ of this special-anniversary event ( All accepted papers will be published in a special issue of Notulae Botanicae Horti Agrobotanici Cluj-Napoca journal.