SHST - Horticulture and Forestry Society from Transylvania, 40 Years of Horticulture Education in Cluj-Napoca

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Antioxidant Capacity of Some Romanian and Cypriot Wines: Spectrophotometrical Approach

Last modified: 2017-04-18


Free radicals have an important role in food and in chemical material degradation, contributing to the occurrence of many human health problems. Highly reactive free radicals and oxygen species existing in biological systems can oxidize nucleic acids, proteins and lipids, starting degenerative diseases. Antioxidants can considerably delay or prevent the oxidation of easily oxidable substrates. Plants contain high concentrations of numerous bioactive constituents with antioxidant activity such as polyphenols. Flavonols, flavones, anthocyanidins and flavanols are reported to be found in significant quantities in wines. The experimental work involves the determination of antioxidant activity, expressed by the presence of these compounds, in several Romanian and Cypriot wines. In this paper work, the antioxidant activity quantification was carried out by the DPPH method. This spectrophotometric method is fast, simple and cheap and is based on the absorbance decrease determination of the DPPH• radical (2,2-diphenyl-1-picrylhydrazyl) in the presence of antioxidants. DPPH• is characterized as a stable free radical due to unpaired electron delocalization over the entire molecule. The antioxidant activity of the wine is thus defined by the dilution of wine required to decrease the initial concentration of DPPH• by 50%: Effective Dilution = ED50. Under these conditions, the lower ED50 of a tested samples, the higher its antioxidant activity. It was analyzed commercial and experimental wines. The highest antioxidant activity for white wines was determined at ‘Lemesos Spouriko’ wine from 2013 (ED50=1/38) and for red wines, at ‘Fetească neagră’ wine from 2014 (ED50=1/590).



This abstract was accepted to be presented at the conference entitled “40 Years of Horticulture Education in Cluj-Napoca” Cluj-Napoca, September 27, 2017, being included in ‘Book of Abstracts’ of this special-anniversary event ( All accepted papers will be published in a special issue of Notulae Botanicae Horti Agrobotanici Cluj-Napoca journal.