SHST - Horticulture and Forestry Society from Transylvania, 40 Years of Horticulture Education in Cluj-Napoca

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Biological Control of Fusarium oxysporum Using Trichoderma spp. on Soil and Rockwool Substrates in Controlled Environment
Radu Mircea GIURGIU, Adelina DUMITRAȘ, Gavrilă MORAR, Petra SCHEEWE, Fritz G. SCHRÖDER

Last modified: 2017-04-17

Abstract


Medicinal plant cultivation in Controlled Environment (CE) is increasing in the context of the new findings regarding the abiotic stress factors manipulation that leads to a significant increment in bioactive substances. Hypericum perforatum seedlings were inoculated with Fusarium oxysporum and with beneficial microorganism, Trichoderma spp.  The experiment was repeated two times; i) inoculation of the substrate and ii) inoculation of the root system. The experiments were done in randomized blocks with the following variants: 1) Control (no inoculation); 2) Inoculated with Fusarium oxysporum; 3) Inoculated with Trichoderma spp.; 4) Inoculated with both Fusarium oxysporum and Trichoderma spp. The results showed that the method of inoculation has a significant effect on the success of the biological control. The inoculation of Trichoderma spp. is less successful in the sterile substrates due to the difficulty of maintaining an optimal environment for the microorganisms. The plants treated with Trichoderma had a two-fold increase in foliar biomass and better developed roots, than the plants inoculated with the pathogen. Morphologically there were no significant differences, with some exceptions of the leaf surface, highest for the plants inoculated with Fusarium oxysporum. The health status of the seedlings inoculated with the pathogen showed signs of the disease, even in the case of the presence of Trichoderma spp. Finally, chemical determinations of Volatile Organic Compounds (VOCs) at the root level showed that the biotic stress increases the secondary metabolism and though the bioactive substances in the plants, giving new perspectives in the cultivation of medicinal plants in CE.

 

 

This abstract was accepted to be presented at the conference entitled “40 Years of Horticulture Education in Cluj-Napoca” Cluj-Napoca, September 27, 2017, being included in ‘Book of Abstracts’ of this special-anniversary event (http://conference.shst.ro). All accepted papers will be published in a special issue of Notulae Botanicae Horti Agrobotanici Cluj-Napoca journal.